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WG2 Epigenetic Technology

Focus

  • Improvements in analytical techniques for epigenetics e.g. CHIP, proteomics for the characterisation of chromatin with an emphasis on identification and quantification of modifications occurring at a low frequency e.g. oxidised cytosine in DNA.
  • Development and running of high-throughput in vitro assays for testing compounds prepared by WG1.
  • Development of plant and animal in vivo functional models for agrochemical and human health care targets.

Workplan

  1. Synthesis of histone peptide probes bearing diverse modified side chains.
  2. Substrate profiling and sequence selectivity of DNA and histone modifying enzymes.
  3. Improved methods for CHIP and DNA sequencing.
  4. Detection of rare DNA modifications such as 5-formyl and 5-carboxyl cytosine residues.
  5. Epigenome-wide sequencing of disease states.
  6. New diagnostic methods for early epigenetic detection of disease.
  7. High-throughput assays for epigenetic enzymes and protein receptors.
  8. Assays for detection of miRNA changes in expression.
  9. Cell-based high content assays for epigenetic targets.
  10. Animal models for epigenetic targets.

Coordinator

Working Groups Members


Cost UE