- Improvements in analytical techniques for epigenetics e.g. CHIP, proteomics for the characterisation of chromatin with an emphasis on identification and quantification of modifications occurring at a low frequency e.g. oxidised cytosine in DNA.
- Development and running of high-throughput in vitro assays for testing compounds prepared by WG1.
- Development of plant and animal in vivo functional models for agrochemical and human health care targets.
- Synthesis of histone peptide probes bearing diverse modified side chains.
- Substrate profiling and sequence selectivity of DNA and histone modifying enzymes.
- Improved methods for CHIP and DNA sequencing.
- Detection of rare DNA modifications such as 5-formyl and 5-carboxyl cytosine residues.
- Epigenome-wide sequencing of disease states.
- New diagnostic methods for early epigenetic detection of disease.
- High-throughput assays for epigenetic enzymes and protein receptors.
- Assays for detection of miRNA changes in expression.
- Cell-based high content assays for epigenetic targets.
- Animal models for epigenetic targets.
- Prof. Carmen JERONIMO
- Instituto Portugues de Oncologia do Porto
- Rua Dr. Antonio Bernardino de Almeida
- 4200-072 Porto, Portugal
Working Groups Members
Laboratory of Animal Biology, National Research Development Institute for Animal Biology and Nutrition (IBNA)