AddressKoc University School of Medicine, Rumelifeneri Yolu. 34450 Sariyer, IstanbulTurkey
Reprogramming, chromatin modifiers, pluripotency, Induced pluripotent stem cells (iPSCs), disease modelling
primary human fibroblasts, patient samples, transgenic reporter mouse strains to monitor pluripotency
Reprogramming with Lenti- and retro-viral gene transduction, episomal vectors and Nucleofection, ChIP, shRNA and Crispr library screens, derivation of patient- and disease-specific iPSC lines
Collaborations outside COST
We have an ongoing collaboration with Prof. Udo Oppermann (University of Oxford) to test the role of small molecule inhibitors of chromatin modifiers in reprogramming. In addition ongoing collaborations exist with clinicians and geneticists throughout Turkey that allow access to patient samples from rare genetic disorders for iPSC-based disease modeling
Short description of ongoing research projects
Our lab studies the molecular mechanisms of cellular reprogramming. We want to understand how somatic cells such as skin fibroblasts can be converted into induced pluripotent stem cells (iPSC). Our focus is on identifying the role of chromatin modifying proteins in this process through a combination of genetic and chemical screens. We are also interested in generating iPSCs from patients with rare genetic diseases for in vitro disease modeling and genome editing.
- Onder TT, Kar N, Cherry A, Sinha AU, Bernt K, Zhu N, Cahan P, Mancarci O, Unternaehrer J, Gupta PB, Lander ES, Armstrong, Daley GQ. (2012) Chromatin-modifying enzymes as modulators of reprogramming. Nature. 483: 598-602.
- Fidan K, Ebrahimi A, Çağlayan ÖH, Özçimen B, Önder TT. Transgene-Free Disease-Specific iPSC Generation from Fibroblasts and Peripheral Blood Mononuclear Cells. Methods Mol Biol. 2016;1353:215-31.
- Das PP, Shao Z, Beyaz S, Apostolou E, Pinello L, De Los Angeles A, O'Brien K, Atsma JM, Fujiwara Y, Nguyen M, Ljuboja D, Guo G, Woo A, Yuan GC, Onder T, Daley G, Hochedlinger K, Kim J, Orkin SH. Distinct and combinatorial functions of Jmjd2b/Kdm4b and Jmjd2c/Kdm4c in mouse embryonic stem cell identity. Mol Cell. 2014 Jan 9;53(1):32-48
- Zhu J, Adli M, Zou JY, Verstappen G, Coyne M, Zhang X, Durham T, Miri M, Deshpande V, De Jager PL, Bennett DA, Houmard JA, Muoio DM, Onder TT, Camahort R, Cowan CA, Meissner A, Epstein CB, Shoresh N, Bernstein BE. (2013) Genome-wide Chromatin State Transitions Associated with Developmental and Environmental Cues. Cell. 152(3):642-54.
- Onder TT and Daley GQ. (2012) New lessons learned from disease modelling with induced pluripotent stem cells. Curr Opin Genetics Dev. Oct;22(5):500-8.
Other activities of potential interest to others
Member of the EMBO Young Investigator Programme - Installation Grantee