polyphenols, absorption, immune response genes expression, pig as animal model, oxidative stress
intestinal cell lines, pig ( blood, plasma, tissue, urine), cow ( blood, plasma,milk). TARGETS: effect/interaction of environmental, nutrition and genetic factors on the health and immune parameters of studied models
Real time PCR for gene expression of different biomarkers (cytokines, enzymes, signaling mollecules, proteins etc) of metabolic and physiologic processes from cells and tissues (blood,organs, muscle, milk), biochemistry (ELISA for cytokines, immunoglobulins, ROS production, enzymes activity, total polyphenols, UV-Vis spectroscopy, antioxidant activity), cell culture (insert techniques, MTT, NO)
The team is involved in several European project such as: FP6 (FOOD -CT40-2004-506144; FEEDSEG-CT-2008-043077) and FP7 (FEED-TO-FOOD–CT-2008-212110; SOLID-CT-2010-266367, FOODSEG-CT-2011-266061), COST (FEED FOR HEALTH-FA0802, Pigunet-FA1401), ECO-NET, bilateral projects (France-BRANCUSI, Greece, Vietnam) as well as national projects.
COST Action FA1401 – PiGutNet: Increase the knowledge about the effect/interaction of environmental, nutrition and genetic factors on the composition of the microbiota in the gastrointestinal tract of pigs and to improve the risk management associated with antibiotic resistance in pig production PNII ID PCE 2011-3-0339 (IDEA 101): Studiyng the toxicity if zearalenone, natural contaminat of feed. Using new tools of genomics and proteomics (PCR microarrays, real time PCR, western blot), the project aims to obtain a better insight in the mechanisms of action of Zearalenone, a Fusarium mycotoxin and the changes produced by this toxin in pig immune response genes expression and related signalling pathway. PN-II-PT-PCCA-2011-3.1-0616: Impact of feed co-contamination and mitigating solutions to increase feed safety, animal health and food quality. The main objective of the project is to investigate the impact of several feed contaminants ( e.g. microorganisms and mycotoxins) when they are present in combination (co-contamination) on: (i) animal performance, (ii) health (intestinal barrier and immune response), (iii) stress responses, (iv) liver function and metabolism. PN-II-RU-TE-2012-3-0086: Role of ochratoxin A in triggering the Balkanic endemic nephropathy using pig as animal model. The general objective of this project relies on two important and original arguments: i) the necessity to go deeper with the studies regarding the involvement of ochratoxin A in endemic nephropathy by the investigating the mechanisms of action of this mycotoxin at the kidney level (cellular and molecular-genetic) which are not yet well defined and are controversial. PN-II-RU-TE-2012-3-0048: Valorization of wine industry waste as a source of antioxidants for an enriched animal diet.The main objective of this project is to evaluate the effects of grape pomace rich in polyphenols on piglets’ performance, antioxidant activity of dietary grape pomace on health and immune parameters.