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Kyselovic group.

kyselovic@uniba.sk
www.fpharm.uniba.sk/en/divisions/department-of-pharmacology-and-toxicology/
Address
Prof Jan KYSELOVIC. Comenius University Bratislava. Faculty of Pharmacy. Odbojarov 10. 832 32 Bratislava. Slovakia.
Slovakia
Keywords

heart failure; signal pathways; epigenetics; transcriptional factors; miRNAs

Models

Explanted human heart, cell lines, patient samples (blood, plasma, tissue), experimental animal models of cardiovascular diseases, Hypertension, diabetes mellitus, cardiac hypertrophy and schema, heart failure

Techniques

Gene expression (RT-PCR, Western blotting), histology and morphology, confocal microscopy, cell cultivation, cytometry, pharmacological assays, biochemistry assays, isolated organs, in situ cardiovascular fysiology.

Collaborations outside COST

We participate in the heart transplantation program, cell based therapy of advanced critical limb ischemia and myocardium after infarction at The National Institute of Cardiovascular Diseases NUSCH a.s. International cooperation: Prof. Wilhem Schimtz, Prof. Frank Muller: Instutute Pharmackologie und Toxikologie, Munster Universitet, Germany Prof. Balwant Tuana, Dept. Molecular Medicine, Ottawa University, Canada Prof.. Robert H. Henning, Dept. Pharmacology, Univerzity of Gronningen, Holland Prof. Peter Ferdinandy, Dept. Pharmacology, Faculty of Medicine, Semmelweis University, Budapest, Hungary

Short description of ongoing research projects

Proposal of project There are several challenges to the translation of these new findings into cardiac regenerative therapies. The proposed project is focused to evaluation the possible specific synergic pharmacological intervention to signal pathways of epigenetics and transcriptional factors and miRNAs in heart failure: - response for activation of foetal gene program, myosine switching and/or hypertrophy process to inhibit the pathoethiology of heart failure, - reprogram of cardiac fibroblasts into cardiac-like myocytes in pathological dysregualted myocardium and exert their effects on the heart regenerat ion, - potentiation molecular signals of paracrine activity of multipotent autotransplanted bone marrow derivates that control and regulate regenerative healing of myocardium. In this project we plane to develop the research in the preclinical stage, just before proceeding to a clinical stage. Based on the outcomes of pervious phases and taking into account the general situation of research related to regenerative medicine, we are especially promoting research using human stem cells and related research and development, the outcomes of which will directly contribute to the benefits therapy of cardiovascular diseases.

Publications
  1. Kyselovič, Ján - Martinka, Peter - Baťová, Zuzana - Gažová, Andrea - Godfraind, Theophile : Calcium channel blocker inhibits western-type diet-evoked atherosclerosis development in apoE-deficient mice(S) In: Journal of Pharmacology and Experimental Therapeutics. - Vol. 315, No. 1 (2005), s. 320-328
  2. Křenek, Peter, ml. - Kmecová, Jana - Kučerová, Dana - Bajuszová, Zuzana - Musil, Peter - Gažová, Andrea - Ochodnický, Peter - Klimas, Ján - Kyselovič, Ján: Isoproterenol-induced heart failure in the rat isassociated with nitric oxide-dependent functional alterations of cardiac function In: European Journal of Heart Failure. - Vol. 11, No. 2 (2009), s. 140-146
  3. Vaja, Václav - Ochodnický, Peter - Křenek, Peter, ml. - Klimas, Ján - Bajuszová, Zuzana - Kyselovič, Ján: Rapid large artery remodeling following the administration and withdrawal of calcium channel blockers in spontaneouslyhypertensive rats In: European Journal of Pharmacology. - Vol. 619, No. 1-3 (2009), s. 85-91
  4. Ochodnicky Peter - Mesarosova Lucia - Cernecka Hana - Klimas Jan - Krenek Peter - Goris M.- van Dokkum RP -Henning RH - Kyselovic Jan. Pioglitazone, a PPARγ agonist, provides comparable protection to angiotensin converting enzyme inhibitor ramipril against adriamycin nephropathy in rat. Eur J Pharmacol. 2014 May 5;730:51-60
  5. Salmikangas P, Menezes-Ferreira M, Reischl I, Tsiftsoglou A, Kyselovic J, Borg JJ, Ruiz S, Flory E, Trouvin JH, Celis P, Ancans J, Timon M, Pante G, Sladowski D, Lipnik-Stangelj M, Schneider CK Manufacturing, characterization and control of cell-based medicinal products: challenging paradigms toward commercial use. Regen Med. 2015 Jan;10(1):65-78.
Other activities of potential interest to others


Cost UE