Department of Biological DNA Modification, Klimasauskas group.

Address

JGMC, Vilnius University Institute of Biotechnology, Saulėtekio al. 7, LT-10257 Vilnius
Lithuania

Keywords

DNA methylation, methyltransferase mechanism and engineering, synthetic analogues of AdoMet, new methods for epigenome analysis, non-coding RNA

Models

epigenomic tools, DNA methylation, methyltransferase mechanism and engineering

Techniques

Targeted covalent labeling of DNA/RNA, DNA methylome profiling, NGS, enzyme engineering, synthesis of AdoMet analogues, miRNA analysis, HPLC-MS

Collaborations outside COST

Rob Neely/Johan Hofkens, Single molecule genotyping/ KU Leuven, Belgium Art Petronis, Epigenome studies/Toronto U., Canada Matthias Bochtler/ DNA demethylation mechanisms, IIMCB, Warsaw, Poland Guoliang Xu/ DNA methylation demethylation, SIBS Shanghai, China

Short description of ongoing research projects

Molecular tools for genome-wide profiling of epigenetic marks (DNA/RNA methylation). Single-cell temporal tracking of DNMT action in live cells (ERC). Cellular regulation involving non-coding RNAs. Biosynthetic selenoproteins with genetically-encoded photocaged selenocysteines

Publications

Liutkevičiūtė Z., Lukinavičius G., Masevičius V., Daujotytė D. and Klimašauskas S. Cytosine-5 methyltransferases add aldehydes to DNA. Nat. Chem. Biol. 2009, 5: 400-402.
Kriukienė E., Liutkevičiūtė Z.and Klimašauskas S. 5-Hydroxymethylcytosine, a new epigenetic mark in mammalian DNA. Chem. Soc. Rev. 2012, 19: 6916–6930.
Kriukienė E., V. Labrie, T. Khare, G. Urbanavičiūtė, A. Lapinaitė, K. Koncevičius, D. Li, T. Wang, S. Pai, C. Ptak, J. Gordevičius, S.-C. Wang, A. Petronis and S. Klimašauskas. DNA unmethylome profiling by covalent capture of CpG sites. Nat. Commun. 2013: 4, 2190.
Liutkevičiūtė, Z., E. Kriukienė, J. Ličytė, M. Rudytė, G. Urbanavičiūtė and S. Klimašauskas. Direct decarboxylation of 5-carboxylcytosine by DNA C5-methyltransferases. J. Am. Chem. Soc., 2014, 136: 5884−5887.
Staševskij, Z., Gibas, P., Gordevičius, J., Kriukienė, E., and Klimašauskas, S. Tethered Oligonucleotide-Primed sequencing, TOP-seq: a high resolution economical approach for DNA epigenome profiling. Mol. Cell, 2017, 65(3): 554–564.