info@epichembio.eu + 34 986 812 316

Plant Functional Genomics Group, Malhó group

aafigueiredo@fc.ul.pt
bioisi.ciencias.ulisboa.pt/node/15
Address
Faculdade de Ciências da UL, Edificio C2, 2.4.49, Campo Grande, 1479-016 Lisboa
Portugal
Keywords

Systems biology, ‘Omics, proteases, plant-pathogen interactions, grapevine

Models

Vitis vinifera (grapevine)

Techniques

• Transcriptomics: microarray, RNA-seq, real time PCR • Proteomics: 2D-DIGE, shotgun proteomics, proteogenomics • Metabolomics: untargeted metabolomics; pathway analysis • Biochemistry: recombinant protein expression and purification

Collaborations outside COST

My group is dedicated to the study of plant-pathogen interactions through a systems biology approach. We have been focused on several ‘Omics approaches (transcriptomics, metabolomics, proteomics) that were combined in an integrative analysis to exploit processes and mechanism linked to plant resistant to biotic stressors. In the plant-pathogen field we collaborate with several national international groups, namely Dr. Günther Buchholz, Head of grapevine biotechnology department at RLP AgroScience/AlPlanta-Institute for Plant Research; Dr. Eva Zyprian at the Institute for grapevine breeding - Julius Kühn-Institut (JKI) Federal Research Centre for Cultivated Plants; Dr. Maria Céu Machado at Centro de Investigação das Ferrugens do Cafeeiro (CIFC) -Instituto Superior de Agronomia (ISA) - Universidade de Lisboa (ULisboa); Dr. Jorge Cunha at the Portuguese Grapevine Ampleographic field - INIAV. In the MS, proteomics and metabolomics field, we collaborate with several research groups, namely Prof. Peter Roepstorff’s at Southern Denmark University; Prof. Carlos Cordeiro and Dr. Marta Sousa Silva at Science Faculty of Lisbon University; Dr. Ana Varela Coelho at ITQB/Universidade Nova de Lisboa and Prof. Robert Verpoorte at Leiden University. We also collaborate with other research groups on recombinant protein expression (Dr. Rita Abranches at ITQB/Universidade Nova de Lisboa and Prof. Andreas Schaller at Hohenheim University (specialist on plant subtilases) and bioinformatics within Lisbon University.

Short description of ongoing research projects

Our first approach was to perform a high throughput analysis using several ‘Omics to characterize grapevine resistance mechanisms towards pathogenic fungi. We have identified resistance associated candidates and mechanisms. Within the resistance associated candidates we have identified a serine protease (subtilase) that may be linked to immune priming events. We have further characterized grapevine subtilase gene family and are currently analyzing these candidates by recombinant protein production, expression analysis and epigenetic control. Moreover, we are also analyzing lipid associated signalling events (lipidomics analysis by Gas chromatography, thin layer chromatography, FTICR-MS) on the grapevine-fungi pathosystem and have correlated these events with subtilases. We have also other projects on the characterization of the metabolome modulation in grapevine resistance to biotic stress.  TARGETS: Resistance-associated genes, proteins, metabolites

Publications
  1. Figueiredo J, Sousa Silva M, Figueiredo A (2017) Subtilisin-like proteases in plant defence: the past, the present and beyond. Molecular Plant Pathology. doi: 10.1111/mpp.12567
  2. Diniz I, Figueiredo A, Loureiro A, Batista D, Azinheira H, Várzea V, Pereira AP, Gichuru E, Moncada P, Guerra-Guimarães L, Oliveira H, Silva MC. (2017). A first insight into the involvement of phytohormones pathways in coffee resistance and susceptibility to Colletotrichum kahawae. PLoS ONE 12(5): e0178159. https://doi:10.1371/journal.pone.0178159
  3. Figueiredo J, Maia M, Sousa Silva M, Figueiredo A (2017) Grapevine subtilase family: update on new sequences and nomenclature proposal. Frontiers in Plant Science, 8: 716; doi: 10.3389/fpls.2017.00716
  4. Figueiredo A, Martins J, Sebastiana M, Guerreiro A, Silva A, Matos AR, Monteiro F, Pais MS, Roepstorff P, Coelho AV (2017) Specific adjustments in grapevine leaf proteome discriminating resistant and susceptible grapevine genotypes to Plasmopara viticola. Journal of Proteomics, 152: 48–57
  5. Sebastiana M, Martins J, Figueiredo A, Monteiro F, Sardans J, Penuelas J, Silva A, Roepstorff P, Pais MS, Coelho AV (2017) Oak protein profile alterations upon root colonization by na ectomycorrhizal fungus. Mycorrhiza: DOI 10.1007/s00572-016-0734-z
Other activities of potential interest to others

Our first approach was to perform a high throughput analysis using several ‘Omics to characterize grapevine resistance mechanisms towards pathogenic fungi. We have identified resistance associated candidates and mechanisms. Within the resistance associated candidates we have identified a serine protease (subtilase) that may be linked to immune priming events. We have further characterized grapevine subtilase gene family and are currently analyzing these candidates by recombinant protein production, expression analysis and epigenetic control. Moreover, we are also analyzing lipid associated signalling events (lipidomics analysis by Gas chromatography, thin layer chromatography, FTICR-MS) on the grapevine-fungi pathosystem and have correlated these events with subtilases. We have also other projects on the characterization of the metabolome modulation in grapevine resistance to biotic stress.


Cost UE