gene targeting, epigenetic writers, epigenetic erasers, Artificial Transcription Factors, gene therapy
cancer cell lines, primary cell lines (fibroblast, ovarian epithelial), patient samples
state-of-the-art Zinc Finger engineering/CRISPR, Gene-targeted Epigenetic Overwriting, FACS facility (Mo-Flow sorting), pyrosequencing, DNA hydroxymethylation, ChIP(seq), (retro)viral transductions, (cell targeted) protein delivery
My lab collaborates with medical doctors (Oncology, Nephrology) for animal models and patient material (tissue slice technology), with biologists on fundamental functional chromatin biology and on various other pathophysiological pathways (asthma, obesity), with chemists (epidrugs, oxidative stress) and with a SME on (targeted) delivery of chemicals, DNA, (si)RNA, proteins (Synvolux Therapeutics Inc, Groningen)
Sustained tumor suppressor gene re-expression, sustained oncogene silencing, permanent modulation of pro- and anti-fibrosis gene expression (regenerative medicine, kidney transplantation) by targeting DNA (de)methylating enzymes and histone writers/erasers to genomic loci using Zinc Finger engineering and CRISPRdCAs targeting; Mitochondrial epigenetics
Associate editor Clinical Epigenetics, Research coordinator ITN EpiPredict (www.EpiPredict.eu) (recruiting students April 2015)