info@epichembio.eu + 34 986 812 316

Tiziana Brevini Group. Stem Cell Differentiation

tiziana.brevini@unimi.it
users2.unimi.it/unistem/
Address
Via Celoria 10, Milan 20133
Italy
Keywords

Stem cells, DNA methylation, epigenetic conversion, translational medicine, cell commitment and differentiation

Models

Primary cell lines, mice models, patient samples

Techniques

- Derivation of stem cell lines, both adult and asymmetrically imprinted ones. Characterization through confocal microscopy, SEM, TEM, Genome-wide characterization, Methylation profiling, Functional analysis through ELISA and protein analysis - In vitro systems for genetic alterations/modifications: siRNA, CRISPR Cas - In vivo murine models (SCID, NOD, LUC mice), Mechanosensing (hydrogels and scaffold homing)

Collaborations outside COST

We are in an institution devoted to translational research in biomedicine. We therefore reinforce collaboration with clinicians from local and national Hospitals as well as international Medical Centers (mainly diabetology but not restricted to). We also collaborate with experts in bioengineering, trying to identify the most adequate substrate that may guarantee an optimal cell homing at the engraft site. Different SMEs help us with innovation and the technological transfer of our research.

Short description of ongoing research projects

Current research focus on the derivation of cell lines generated through the exposure to epigenetic modifiers, with the aim to obtain phenotype switch without the use of retroviral vectors, nor the acquisition of a stable pluripotent state. We have a specific interest in understanding the epigenetic mechanisms driving cell commitment and cell plasticity. We have specific projects in type 1 and 2 diabetes and rare disorders related to monogenic syndromes.

Publications
  1. Phenotype switching through epigenetic conversion. Brevini TA, Pennarossa G, Maffei S, Gandolfi F. Reprod Fertil Dev. 2015 Mar 5. doi: 10.1071/RD14246.
  2. Morphological and molecular changes of human granulosa cells exposed to 5-azacytidine and addressed toward muscular differentiation. Brevini TA, Pennarossa G, Rahman MM, Paffoni A, Antonini S, Ragni G, deEguileor M, Tettamanti G, Gandolfi F. Stem Cell Rev. 2014 Oct;10(5):633-42. doi: 10.1007/s12015-014-9521-4.
  3. Reprogramming of pig dermal fibroblast into insulin secreting cells by a brief exposure to 5-aza-cytidine.Pennarossa G, Maffei S, Campagnol M, Rahman MM, Brevini TA, Gandolfi F. Stem Cell Rev. 2014 Feb;10(1):31-43. doi: 10.1007/s12015-013-9477-9.
  4. Brief demethylation step allows the conversion of adult human skin fibroblasts into insulin-secreting cells. .Pennarossa G, Maffei S, Campagnol M, Tarantini L, Gandolfi F, Brevini TA. Proc Natl Acad Sci U S A. 2013 May 28;110(22):8948-53. doi: 10.1073/pnas.1220637110.
  5. Centrosome amplification and chromosomal instability in human and animal parthenogenetic cell lines. Brevini TA, Pennarossa G, Maffei S, Tettamanti G, Vanelli A, Isaac S, Eden A, Ledda S, de Eguileor M, Gandolfi F. Stem Cell Rev. 2012 Dec;8(4):1076-87. doi: 10.1007/s12015-012-9379-2.
Other activities of potential interest to others

• Editorial Board of Stem Cells Review and Report • MC Member of the COST Action “Epiconcept” FA 1201 • MC Member of the COST Action SAALAM BM1308


Cost UE