info@epichembio.eu + 34 986 812 316

Portuguese Mass Spectrometry Network & GEMAB Group & Enzymology Group

apamarques@fc.ul.pt
http://ftms.fc.ul.pt/ & http://cqb.fc.ul.pt/research/environmental-biological-mass-spectrometry/ & enzymology.fc.ul.pt
Address
Faculdade de Ciências da Universidade de Lisboa, Campo Grande, Edifício C8, 1749-016 Lisboa, Portugal
Portugal
Keywords

Mass spectrometry, metabolites, small molecules

Models

Leishmania infantum, Vitis vinifera, fungus, several samples (ex. teas and organic samples)

Techniques

Gas Chromatography/Mass Spectrometry, Liquid Chromatography/Mass Spectrometry, Fourier Transform Ion Cyclotron Resonance/Mass Spectrometry

Collaborations outside COST

The GEMAB group has collaborations with Magda Claeys´s at Atwerpen Universiteit and Jaap Boon at Jaap Enterprises. The main goal of this group is to explore the potentialities of advanced mass spectrometry to investigate at molecular level, the structure, and the reactivity of compounds with environmental and biological interest. The Enzymology group has several collaborations with Neil Kelleher’s at Northwestern University, Peter Roepstorff’s at Southern Denmark University, Roman Zubarev’s at Karolinska Institute and Albert Heck’s at Bijvoet Center. The mission of this group is to unravel enzyme function and structure, exploring the exquisite complexity of life through a systems biology approach. Both groups also collaborate with some pharmaceutical industries and other groups in Universidade de Lisboa.

Short description of ongoing research projects

The projects consist in the identification of metabolites using ultra-high resolution and high-mass accuracy provided by FTICR-MS. The models used for these projects are Human visceral leishmaniasis (an important public health problem) and grapevine downy-mildew (a major economical concern). The metabolites identified will allow to build a network of metabolic pathways that will reveal the pathways and the mechanisms responsible for the infection and the key metabolites associated with resistance and survival.

Publications
  1. S. G. Santos, A. P. Marques, D. L. D. Lima, D. V. Evtuguin and V. I. Esteves. Kinetics of Eucalypt Lignosulfonate Oxidation to Aromatic Aldehydes by Oxygen in Alkaline Medium. Industrial & Engineering Chemistry Research, vol. 50, 291-298 (2011).
  2. A. P. Marques, D. V. Evtuguin, S. Magina, F. M. L. Amado and A. Prates. Chemical Composition of Spent Liquors from Acidic Magnesium-Based Sulphite Pulping of Eucalyptus globulus. Journal of Wood Chemistry and Technology, vol. 29, 322-336 (2009).
  3. A. Cavaleiro, A. P. Marques, J. V. Fernandes, N. J. M. Carvalho and J. M. Th. De Hosson. Evolution of the Microstructure, Residual Stresses and Mechanical Properties of W-Si-N Coatings after Thermal Annealing. Journal of Materials Research, vol. 20, 1-13 (2005).
  4. A. P. Marques and A. Cavaleiro. Structural and Mechanical Properties of Amorphous W-Si-N Sputtered Films after Thermal Annealing. Thin Solid Films, vol. 441, 150-160 (2003).
  5. A. P. Marques, C. M. A. Brett, H. D. Burrows, A. P. Monkman and B. Retimal. Spectral and Electrochemical Studies on Blends of Polyaniline and Cellulose Esters. Journal of Applied Polymer Science, vol. 86, 2182-2188 (2002).
Other activities of potential interest to others

Team member of the Rede Nacional de Espectrometria de Massa.


Cost UE