Histone variants, drug response, metabolism, cancer, myelodysplastic syndrome
Primary and established cell lines, mouse xenografts, zebrafish, patient samples
Cell culture and manipulation including primary cells and embryonic stem cells, differentiation and transformation protocols, viral gene cassette transduction, Chromatin IP in all its facets including ChIP-seq, epigenomic and transcriptomic data mining, bioinfomrmatics, FISH, affinity purifications coupled to mass spectrometry, basic biochemistry.
My lab has a large number of established collaborations to complement our capacities with mouse models, zebrafish, sub-diffraction microscopy and metabolomics among others. Clinical collaborators further provide access to primary samples primarily from myeloid dysplastic syndrome and squamous cell carcinoma patients.
Epigenetic information is written in chromatin. But how exactly do chromatin alterations contribute to cell fate transitions? How does the environment influence these processes? And how does the metabolic state of a cell impact on its chromatin structure and its epigenetic memory? These are questions we address in the lab. Current projects foscus on the histone variant macroH2A and chromatin modifiers that contribute to colorectal cancer and myelodysplastic syndrome.
Organiser of the Barcelona Conference Series on Epigenetics and Cancer. Registration for BCEC 2015 on October 29+30 here: http://www.bdebate.org/en/forum/coding-and-non-coding-functions-genome-2015-bcec