enhancers, plasma cells, myeloma, transcription, germ cells
mouse ES cells, human plasma cells, myeloma, mouse germ cells
ChIPseq, RNAseq, low cell number ChIP, ES cell differentiation to plasma cells, CRISPR-Cas9
Thorunn Rafnar, deCODE genetics, Iceland, Unnur Thorsteinsdóttir, deCODE genetics, Iceland Petra Hajkova, Imperial College London, UK Elizabeth Robertson, Oxford University, UK
We have two ongoing research objectives in my laboratory, both stemming from my experience with working with the transcription factor BLIMP1 which is both an inducer of plasma cell fate and primordial germ cell fate in mouse and human. We are currently investigating the relationship between BLIMP1 and polycomb repressive complex 2 in myeloma, as well as primordial germ cells. Additionally we are interested in cis-regulatory elements directing cellular states, and are profiling enhancers in purified plasma cells from myeloma patient bone marrow aspirates. We have set up the chromosome conformation capture technique in order to investigate long range chromatin fibre interactions on potential enhancers coming out of our profiling. Finally, we are aiming to expand our studies of mouse primordial germ cells by investigating the role of homeobox transcription factors in their biology, by using the CRISPR-Cas9 system in embryonic stem cells that we then differentiate into primordial germ cells.