info@epichembio.eu + 34 986 812 316

Magnusdottir Laboratory. Transcriptional Control of Cell States

erna@hi.is
lifvisindi.hi.is/staff/erna-magnusdottir
Address
Vatnsmyrarvegur 16, 101 Reykjavik, Iceland
Iceland
Keywords

enhancers, plasma cells, myeloma, transcription, germ cells

Models

mouse ES cells, human plasma cells, myeloma, mouse germ cells

Techniques

ChIPseq, RNAseq, low cell number ChIP, ES cell differentiation to plasma cells, CRISPR-Cas9

Collaborations outside COST

Thorunn Rafnar, deCODE genetics, Iceland, Unnur Thorsteinsdóttir, deCODE genetics, Iceland Petra Hajkova, Imperial College London, UK Elizabeth Robertson, Oxford University, UK

Short description of ongoing research projects

We have two ongoing research objectives in my laboratory, both stemming from my experience with working with the transcription factor BLIMP1 which is both an inducer of plasma cell fate and primordial germ cell fate in mouse and human. We are currently investigating the relationship between BLIMP1 and polycomb repressive complex 2 in myeloma, as well as primordial germ cells. Additionally we are interested in cis-regulatory elements directing cellular states, and are profiling enhancers in purified plasma cells from myeloma patient bone marrow aspirates. We have set up the chromosome conformation capture technique in order to investigate long range chromatin fibre interactions on potential enhancers coming out of our profiling. Finally, we are aiming to expand our studies of mouse primordial germ cells by investigating the role of homeobox transcription factors in their biology, by using the CRISPR-Cas9 system in embryonic stem cells that we then differentiate into primordial germ cells.

Publications
  1. Magnúsdóttir E. Surani M.A. How to make a primordial germ cell. Development 2014 141 (2):245-252.
  2. Magnúsdóttir E., Dietmann S., Murakami K., Gunesdogan U., Tang F., Bao S., Diamanti E., Trotter M., Lao K., Gottgens B., Surani M.A. A tripartite transcription factor network regulates primordial germ cell specification in mice. Nature Cell Biology, 2013 15 (8):905-915.
  3. Grabole N., Tischler J., Leitch H.G., Hackett, J. Kim S., Tang F., Magnúsdóttir E. and Surani M.A. Prdm14 promotes germline fate and naïve pluripotency by modulating signalling and the epigenome. EMBO Reports, 2013 14(7):629-637.
  4. Magnúsdóttir E., Gillich A., Grabole N., Surani M.A. Combinatorial Control of Cell Fate and Reprogramming in the Mammalian Germ Line. Curr. Opin. Genet. Dev. 2012 22(5):466-474.
  5. Gillich A., Bao S., Hayashi K., Trotter M., Grabole N., Pasque V., Magnúsdóttir E., Surani M.A. Epiblast Stem Cell-Based System Reveals Reprogramming Synergy of Germ Line Factors. Cell Stem Cell, 2012 10(4):425-39.
Other activities of potential interest to others


Cost UE