Address
Jagtvej 160, 3rd floor, office C. 327.DenmarkKeywords
Chemical Biology, Histone Demethylases, Histone methylatransferases, Phage Display and assay development
Models
Techniques
Phage display, ELISA, Peptide chemistry, LANCE
Collaborations outside COST
SGC, Promega, Novo Nordisk, Instituto Clodomiro Picado Costa Rica, National Aquarium of Denmark, Danish School of Conservation, Technical University of Denmark,
Short description of ongoing research projects
Development of biomarkers for PRMTs in cells
Development of macrocyclic inhibitors for KDM
Development of macrocyclic inhibitors for EHMTs
Development of assays for screening epigenetic compound tools
Publications
- Kanchan Devkota, Brian Lohse, Camilla Nyby Jakobsen, Jens Berthelsen, Rasmus Prætorius Clausen A FRET-based method for Histone Methyltransferases. Analytical Biochemistry (DOI:10.1016/j.ab.2015.02.012) 2015
- Ulrike Leurs, Brian Lohse*, Kasper D. Rand, Shonoi Ming, Erik S. Riise, Philip A. Cole, Jesper L. Kristensen and Rasmus P. Clausen: Substrate- and Cofactor-independent Inhibition of Histone Demethylase KDM4C. ACS Chem. Biol. (dx.doi.org/10.1021/cb500374f) 2014.
- U. Leurs, B. Lohse, S. Ming, P.A. Cole, J. L. Kristensen and K.D. Rand: Dissecting the Binding Mode of Low AffinityPhage Display Peptide Ligands to Protein Targets by Hydrogen/ Deuterium Exchange Coupled to Mass Spectrometry.Anal Chem. (DOI: 10.1021/ ac503137u) 2014
- K. Devkota, B. Lohse, Q. Liu, M-W. Wang, D. Stærk, J. Berthelsen: Analogues of the Natural Product Sinefungin as Inhibitors of EHMT1 and EHMT2, ACS Med. Chem. Lett., Vol. 5 (4) 293, 2014.
- B. Lohse*, Charlotte Helgstrand, Jan B. L. Kristensen, Ulrike Leurs, Paul A.C. Cloos, Jesper L. Kristensen and RasmusP. Clausen: Posttranslational modifications of the histone 3 tail and their impact on the activity of histone lysinedemethylases in vitro., PLoS ONE vol. 8 (7) 2013.
Other activities of potential interest to others
EpiDiscoverY is a Contract Research based Organization (CRO) under ILF-SUND at the University of Copenhagen. Our main focus is epigenetics and phage display, searching for novel ligands that can be used as compound tools or scaffolds that have the potential to be further developed into biopharmaceuticals. EpiDiscoverY also develop customized assays for specific targets and do screening of costumer targets with our in-house libraries. We have experience working with both industry and academia and have experience with patents, MTA’s and other types of contract, but are also carrying out classical academic research collaborations. A common denominator for all of the projects is the use of phage display technology.
