info@epichembio.eu + 34 986 812 316

Chromatin Molecular Complexes, CEITEC and Faculty of Science, Masaryk University Brno

fojtova@sci.muni.cz
www.ceitec.eu/ceitec-mu/chromatin-molecular-complexes/rg51
Address
Kamenice 5, 625 00 Brno
Czech Republic
Keywords

DNA methylation, histone modifications, telomere, telomerase, chromatin

Models

common plant models (Arabidopsis thaliana, Nicotiana tabacum), plant cultures (BY-2)

Techniques

DNA methylation (methylation sensitive enzymes, BGS), ChIP (qPCR, hybridization, seq), telomerase activity, expression analyses, plant transformation, handling with plant cell cultures including regeneration of plants

Collaborations outside COST

The laboratory has collaborations with scientific institutions in the Czech Republic, e.g., Institute of Biophysics AS CR – group of Biophysical Chemistry and Molecular Oncology, analysis of DNA modifications using biophysical and biochemical methods; Institute of Experimental Botany AS CR – group of Pollen biology, regulation of gene expression in pollen; Institute of Organic Chemistry and Biochemistry AS CR – group of Bioorganic and Medicinal Chemistry, development of biologically active analogues of nucleic acids; the University Hospital Brno – group of Hereditary Genetic Diseases, molecular and genetic diagnostics of neuromuscular and metabolic diseases; and abroad, e.g., Queen Mary University of London, UK – plant telomeres evolution; IPK Gatersleben, Germany – chromatin structure; Hong Kong University of Science & Technology, Hong Kong Special Administrative Region, People’s Republic of China – telomeres in algae.

Short description of ongoing research projects

Epigenetic properties of plant telomeres (structure of telomeric chromatin in epigenetically modulated plants and calli), novel methods for detection of methylation of plant telomeric cytosines, role of telomeres in chromosome stability, mechanisms involved in developmental regulation of plant telomerase, proteins associated with plant telomeres.

Publications
  1. Ogrocká, A., Polanská, P., Majerová, E., Janeba, Z., Fajkus, J., Fojtová, M. Compromised telomere maintenance in hypomethylated Arabidopsis thaliana plants. Nucleic Acids Res., 42, 2919-2931, 2014.
  2. Majerová, E., Mandáková, T., Vu, T.H.G., Fajkus, J., Lysák, M.A., Fojtová, M. Chromatin features of plant telomeric sequences at terminal vs. internal positions. Front Plant Sci., 5, 593, 2014.
  3. Fojtová, M., Fajkus J. Epigenetic regulation of telomere maintenance. Cytogenet. Genome Res., 143, 125-135, 2014. review
  4. Ogrocká, A., Sýkorová, E., Fajkus, J., Fojtová, M. Developmental silencing of the AtTERT gene is associated with increased H3K27me3 loading and maintenance of its euchromatic environment. J. Exp. Bot., 63, 4233-41, 2012.
  5. Ogrocká, A., Sýkorová, E., Fajkus, J., Fojtová, M. Developmental silencing of the AtTERT gene is associated with increased H3K27me3 loading and maintenance of its euchromatic environment. J. Exp. Bot., 63, 4233-41, 2012.
Other activities of potential interest to others

Member of the editorial board, Telomere and telomerase


Cost UE