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Mendel University in Brno, Genetics and Plant Breeding

baranek@mendelu.cz
is.mendelu.cz/lide/clovek.pl?id=3560;interni_vzorek=3560;lang=en
Address
MIGPB FH, Valtická 337, 69144 Lednice
Czech Republic
Keywords

stressed plants; changes of methylome; epigenetic plant breeding; epigenetic writers/erasers

Models

Plant species Nicotiana benthamiana, Petunia hybrida, Vitis vinifera, Prunus spp.

Techniques

Methylation Sensitive Amplified Polymorphism (MSAP); portfolio of different DNA hypomethylating agents working on different principles; development of easy and quick test for evaluation of the DNA demethylating effect; genetically uniform seeds (lines) of different plant species; in vitro plant cultures

Collaborations outside COST

Mendeleum department collaborates with Czech private breeding companies focused on the ornamental plants and vegetables; collaboration with group of chemists preparing new chemical compounds with confirmed or theoretical DNA demethylating effect

Short description of ongoing research projects

Utilisation of epigenetic principles in plant breeding; development of new approaches for easy and quick testing of DNA demethylation effect of chemicals on plants; evaluation of DNA methylation dynamics and reversibility in plants under and after stress

Publications
  1. Baránek, Miroslav -- Křižan, Břetislav -- Raddová, Jana -- Ondrušiková, Eva -- Pidra, Miroslav AFLP-derived methods as a tool for study of genomic, epigenomic and transcriptomic changes in stressed grapevine plants. Acta Horticulturae. 2009. vol. 839, p. 575--583.
  2. Baránek, Miroslav -- Křižan, Břetislav -- Ondrušiková, Eva -- Pidra, Miroslav DNA-methylation changes in grapevine somaclones following in vitro culture and thermotherapy. Plant Cell, Tissue and Organ Culture. 2010. vol. 101, p. 11—22.
  3. Baránek, Miroslav -- Ondrušíková Eva Evaluation of the AFLP and MSAP methods as tools for studying of DNA changes in grapevine plants long period after their in vitro thermotherapy. Acta Horticulturae. 2012. vol. 961, p. 73-80
  4. Mynarzová, Zuzana - Baránek, Miroslav An Effect of Demethylating Agents on Petunia × hybrida Varieties. In The 8th Scandinavian Plant Physiology Society PhD Students Conference.Conference Book. 1. vyd. Uppsala: Swedish University of Agricultural Sciences, 2014, p. 99--100. ISBN 978-91-576-9212-2.
  5. Miroslav Baránek, Jana Čechová, Jana Raddová , Věra Holleinová, Eva Ondrušíková, Miroslav Pidra Dynamics and reversibility of the DNA methylation landscape of grapevine plants (Vitis vinifera) stressed by in vitro cultivation and thermotherapy. PLoS ONE, 14. 5. 2015
Other activities of potential interest to others

Associate editor of “Czech Journal of Genetics and Plant Breeding”. Techniques: Methylation Sensitive Amplified Polymorphism (MSAP); portfolio of different DNA hypomethylating agents working on different principles (we are not the owners, but we can negotiate for you with institute which we collaborate). Models: Plant systems Targets: – in vitro cultures of plants; genetically uniform seeds (lines) of different vegetables and ornamental plants (we are not the owners, but we can negotiate for you with institute which we collaborate)


Cost UE