Stem cells, DNA methylation, epigenetic conversion, translational medicine, cell commitment and differentiation
Primary cell lines, mice models, patient samples
- Derivation of stem cell lines, both adult and asymmetrically imprinted ones. Characterization through confocal microscopy, SEM, TEM, Genome-wide characterization, Methylation profiling, Functional analysis through ELISA and protein analysis - In vitro systems for genetic alterations/modifications: siRNA, CRISPR Cas - In vivo murine models (SCID, NOD, LUC mice), Mechanosensing (hydrogels and scaffold homing)
We are in an institution devoted to translational research in biomedicine. We therefore reinforce collaboration with clinicians from local and national Hospitals as well as international Medical Centers (mainly diabetology but not restricted to). We also collaborate with experts in bioengineering, trying to identify the most adequate substrate that may guarantee an optimal cell homing at the engraft site. Different SMEs help us with innovation and the technological transfer of our research.
Current research focus on the derivation of cell lines generated through the exposure to epigenetic modifiers, with the aim to obtain phenotype switch without the use of retroviral vectors, nor the acquisition of a stable pluripotent state. We have a specific interest in understanding the epigenetic mechanisms driving cell commitment and cell plasticity. We have specific projects in type 1 and 2 diabetes and rare disorders related to monogenic syndromes.
• Editorial Board of Stem Cells Review and Report • MC Member of the COST Action “Epiconcept” FA 1201 • MC Member of the COST Action SAALAM BM1308